Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
Abstract Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the...
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2017-07-01
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doaj-9da294e70b2248cc902b62b89b3556402020-12-08T02:18:14ZengNature Publishing GroupScientific Reports2045-23222017-07-01711910.1038/s41598-017-05025-5Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolatesAida Valmaseda0Quique Bassat1Pedro Aide2Pau Cisteró3Alfons Jiménez4Aina Casellas5Sonia Machevo6Ruth Aguilar7Betuel Sigaúque8Virander S. Chauhan9Christine Langer10James Beeson11Chetan Chitnis12Pedro L. Alonso13Deepak Gaur14Alfredo Mayor15ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaCentro de Investigação em Saúde de Manhiça (CISM)ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaCentro de Investigação em Saúde de Manhiça (CISM)ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaCentro de Investigação em Saúde de Manhiça (CISM)Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB)Macfarlane Burnet Institute for Medical Research and Public HealthMacfarlane Burnet Institute for Medical Research and Public HealthMalaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB)ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaLaboratory of Malaria and Vaccine Research, School of Biotechnology, Jawaharlal Nehru UniversityISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de BarcelonaAbstract Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infection. 11 IRG transcript levels were measured in P. falciparum isolates by RT-PCR, and IgG/IgM against invasion ligands by Luminex®, in 50 Mozambican adults, 25 children with severe malaria (SM) and 25 with uncomplicated malaria (UM). IRG expression differences among groups and associations between IRG expression and clinical/immunologic parameters were assessed. IRG expression diversity was higher in parasites infecting children than adults (p = 0.022). eba140 and ptramp expression decreased with age (p = 0.003 and 0.007, respectively) whereas p41 expression increased (p = 0.022). pfrh5 reduction in expression was abrupt early in life. Parasite density decreased with increasing pfrh5 expression (p < 0.001) and, only in children, parasite density increased with p41 expression (p = 0.007), and decreased with eba175 (p = 0.013). Antibody responses and IRG expression were not associated. In conclusion, IRG expression is associated with age and parasite density, but not with specific antibody responses in the acute phase of infection. Our results confirm the importance of multi-antigen vaccines development to avoid parasite immune escape when tested in malaria-exposed individuals.https://doi.org/10.1038/s41598-017-05025-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aida Valmaseda Quique Bassat Pedro Aide Pau Cisteró Alfons Jiménez Aina Casellas Sonia Machevo Ruth Aguilar Betuel Sigaúque Virander S. Chauhan Christine Langer James Beeson Chetan Chitnis Pedro L. Alonso Deepak Gaur Alfredo Mayor |
spellingShingle |
Aida Valmaseda Quique Bassat Pedro Aide Pau Cisteró Alfons Jiménez Aina Casellas Sonia Machevo Ruth Aguilar Betuel Sigaúque Virander S. Chauhan Christine Langer James Beeson Chetan Chitnis Pedro L. Alonso Deepak Gaur Alfredo Mayor Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates Scientific Reports |
author_facet |
Aida Valmaseda Quique Bassat Pedro Aide Pau Cisteró Alfons Jiménez Aina Casellas Sonia Machevo Ruth Aguilar Betuel Sigaúque Virander S. Chauhan Christine Langer James Beeson Chetan Chitnis Pedro L. Alonso Deepak Gaur Alfredo Mayor |
author_sort |
Aida Valmaseda |
title |
Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates |
title_short |
Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates |
title_full |
Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates |
title_fullStr |
Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates |
title_full_unstemmed |
Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates |
title_sort |
host age and expression of genes involved in red blood cell invasion in plasmodium falciparum field isolates |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-07-01 |
description |
Abstract Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infection. 11 IRG transcript levels were measured in P. falciparum isolates by RT-PCR, and IgG/IgM against invasion ligands by Luminex®, in 50 Mozambican adults, 25 children with severe malaria (SM) and 25 with uncomplicated malaria (UM). IRG expression differences among groups and associations between IRG expression and clinical/immunologic parameters were assessed. IRG expression diversity was higher in parasites infecting children than adults (p = 0.022). eba140 and ptramp expression decreased with age (p = 0.003 and 0.007, respectively) whereas p41 expression increased (p = 0.022). pfrh5 reduction in expression was abrupt early in life. Parasite density decreased with increasing pfrh5 expression (p < 0.001) and, only in children, parasite density increased with p41 expression (p = 0.007), and decreased with eba175 (p = 0.013). Antibody responses and IRG expression were not associated. In conclusion, IRG expression is associated with age and parasite density, but not with specific antibody responses in the acute phase of infection. Our results confirm the importance of multi-antigen vaccines development to avoid parasite immune escape when tested in malaria-exposed individuals. |
url |
https://doi.org/10.1038/s41598-017-05025-5 |
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