Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs
Abstract Increasing evidence suggest that the glucose-lowering drug metformin exerts a valuable anti-senescence role. The ability of metformin to affect the biogenesis of selected microRNAs (miRNAs) was recently suggested. MicroRNA isoforms (isomiRs) are distinct variations of miRNA sequences, harbo...
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doaj-9db56585c5b24fc0af64d8a7a31bcb9d2020-12-13T12:31:45ZengNature Publishing GroupScientific Reports2045-23222020-12-0110111410.1038/s41598-020-78871-5Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRsAngelica Giuliani0Eric Londin1Manuela Ferracin2Emanuela Mensà3Francesco Prattichizzo4Deborah Ramini5Fiorella Marcheselli6Rina Recchioni7Maria Rita Rippo8Massimiliano Bonafè9Isidore Rigoutsos10Fabiola Olivieri11Jacopo Sabbatinelli12Department of Clinical and Molecular Sciences, Università Politecnica Delle MarcheComputational Medicine Center, Thomas Jefferson UniversityDepartment of Experimental, Diagnostic, and Specialty Medicine (DIMES), University of BolognaDepartment of Clinical and Molecular Sciences, Università Politecnica Delle MarcheIRCCS MultiMedicaDepartment of Clinical and Molecular Sciences, Università Politecnica Delle MarcheCenter of Clinical Pathology and Innovative Therapy, IRCCS INRCACenter of Clinical Pathology and Innovative Therapy, IRCCS INRCADepartment of Clinical and Molecular Sciences, Università Politecnica Delle MarcheDepartment of Experimental, Diagnostic, and Specialty Medicine (DIMES), University of BolognaComputational Medicine Center, Thomas Jefferson UniversityDepartment of Clinical and Molecular Sciences, Università Politecnica Delle MarcheDepartment of Clinical and Molecular Sciences, Università Politecnica Delle MarcheAbstract Increasing evidence suggest that the glucose-lowering drug metformin exerts a valuable anti-senescence role. The ability of metformin to affect the biogenesis of selected microRNAs (miRNAs) was recently suggested. MicroRNA isoforms (isomiRs) are distinct variations of miRNA sequences, harboring addition or deletion of one or more nucleotides at the 5′ and/or 3′ ends of the canonical miRNA sequence. We performed a comprehensive analysis of miRNA and isomiR profile in human endothelial cells undergoing replicative senescence in presence of metformin. Metformin treatment was associated with the differential expression of 27 miRNAs (including miR-100-5p, -125b-5p, -654-3p, -217 and -216a-3p/5p). IsomiR analysis revealed that almost 40% of the total miRNA pool was composed by non-canonical sequences. Metformin significantly affects the relative abundance of 133 isomiRs, including the non-canonical forms of the aforementioned miRNAs. Pathway enrichment analysis suggested that pathways associated with proliferation and nutrient sensing are modulated by metformin-regulated miRNAs and that some of the regulated isomiRs (e.g. the 5′ miR-217 isomiR) are endowed with alternative seed sequences and share less than half of the predicted targets with the canonical form. Our results show that metformin reshapes the senescence-associated miRNA/isomiR patterns of endothelial cells, thus expanding our insight into the cell senescence molecular machinery.https://doi.org/10.1038/s41598-020-78871-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angelica Giuliani Eric Londin Manuela Ferracin Emanuela Mensà Francesco Prattichizzo Deborah Ramini Fiorella Marcheselli Rina Recchioni Maria Rita Rippo Massimiliano Bonafè Isidore Rigoutsos Fabiola Olivieri Jacopo Sabbatinelli |
spellingShingle |
Angelica Giuliani Eric Londin Manuela Ferracin Emanuela Mensà Francesco Prattichizzo Deborah Ramini Fiorella Marcheselli Rina Recchioni Maria Rita Rippo Massimiliano Bonafè Isidore Rigoutsos Fabiola Olivieri Jacopo Sabbatinelli Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs Scientific Reports |
author_facet |
Angelica Giuliani Eric Londin Manuela Ferracin Emanuela Mensà Francesco Prattichizzo Deborah Ramini Fiorella Marcheselli Rina Recchioni Maria Rita Rippo Massimiliano Bonafè Isidore Rigoutsos Fabiola Olivieri Jacopo Sabbatinelli |
author_sort |
Angelica Giuliani |
title |
Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs |
title_short |
Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs |
title_full |
Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs |
title_fullStr |
Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs |
title_full_unstemmed |
Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs |
title_sort |
long-term exposure of human endothelial cells to metformin modulates mirnas and isomirs |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-12-01 |
description |
Abstract Increasing evidence suggest that the glucose-lowering drug metformin exerts a valuable anti-senescence role. The ability of metformin to affect the biogenesis of selected microRNAs (miRNAs) was recently suggested. MicroRNA isoforms (isomiRs) are distinct variations of miRNA sequences, harboring addition or deletion of one or more nucleotides at the 5′ and/or 3′ ends of the canonical miRNA sequence. We performed a comprehensive analysis of miRNA and isomiR profile in human endothelial cells undergoing replicative senescence in presence of metformin. Metformin treatment was associated with the differential expression of 27 miRNAs (including miR-100-5p, -125b-5p, -654-3p, -217 and -216a-3p/5p). IsomiR analysis revealed that almost 40% of the total miRNA pool was composed by non-canonical sequences. Metformin significantly affects the relative abundance of 133 isomiRs, including the non-canonical forms of the aforementioned miRNAs. Pathway enrichment analysis suggested that pathways associated with proliferation and nutrient sensing are modulated by metformin-regulated miRNAs and that some of the regulated isomiRs (e.g. the 5′ miR-217 isomiR) are endowed with alternative seed sequences and share less than half of the predicted targets with the canonical form. Our results show that metformin reshapes the senescence-associated miRNA/isomiR patterns of endothelial cells, thus expanding our insight into the cell senescence molecular machinery. |
url |
https://doi.org/10.1038/s41598-020-78871-5 |
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