CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

Type V CRISPR-Cas interference proteins use a single RuvC active site to make RNA-guided breaks in double-stranded DNA substrates, an activity essential for both bacterial immunity and genome editing. The best-studied of these enzymes, Cas12a, initiates DNA cutting by forming a 20-nucleotide R-loop...

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Bibliographic Details
Main Authors: Joshua C Cofsky, Deepti Karandur, Carolyn J Huang, Isaac P Witte, John Kuriyan, Jennifer A Doudna
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-06-01
Series:eLife
Subjects:
CRISPR
genome editing
RNA
deoxyribonuclease
R-loop
Online Access:https://elifesciences.org/articles/55143

Internet

https://elifesciences.org/articles/55143

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