Comparison Between K3EDTA and Lithium Heparin as Anticoagulant to Isolate Bovine Granulocytes From Blood

Comparison Between K3EDTA and Lithium Heparin as Anticoagulant to Isolate Bovine Granulocytes From Blood

Granulocytes play a key role in the defense against invading pathogens. To study granulocyte functions, the isolation of a pure and active cell population from fresh blood is required. Anticoagulants and red blood cells (RBCs) lysis used in the isolation procedure may influence cell harvest, cell ma...

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Bibliographic Details
Main Authors: Shima Hassan Baien, Melissa Natalie Langer, Maike Heppelmann, Maren von Köckritz-Blickwede, Nicole de Buhr
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-07-01
Series:Frontiers in Immunology
Subjects:
granulocytes
anticoagulants
blood
extracellular traps
oxidative burst
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2018.01570/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2018.01570/full

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