Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

Genome editing has now been reported in many systems using TALEN and CRISPR-Cas9 nucleases. Precise mutations can be introduced during homology-directed repair with donor DNA carrying the wanted sequence edit, but efficiency is usually lower than for gene knockout and optimal strategies have not bee...

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Bibliographic Details
Main Authors: Jean-Baptiste Renaud, Charlotte Boix, Marine Charpentier, Anne De Cian, Julien Cochennec, Evelyne Duvernois-Berthet, Loïc Perrouault, Laurent Tesson, Joanne Edouard, Reynald Thinard, Yacine Cherifi, Séverine Menoret, Sandra Fontanière, Noémie de Crozé, Alexandre Fraichard, Frédéric Sohm, Ignacio Anegon, Jean-Paul Concordet, Carine Giovannangeli
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124716301048

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http://www.sciencedirect.com/science/article/pii/S2211124716301048

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