Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis

Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis

Abstract Background Efficient and convenient genome-editing toolkits can expedite genomic research and strain improvement for desirable phenotypes. Zymomonas mobilis is a highly efficient ethanol-producing bacterium with a small genome size and desirable industrial characteristics, which makes it a...

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Bibliographic Details
Main Authors: Wei Shen, Jun Zhang, Binan Geng, Mengyue Qiu, Mimi Hu, Qing Yang, Weiwei Bao, Yubei Xiao, Yanli Zheng, Wenfang Peng, Guimin Zhang, Lixin Ma, Shihui Yang
Format: Article
Language:English
Published: BMC 2019-10-01
Series:Microbial Cell Factories
Subjects:
Zymomonas mobilis
CRISPR
Cas12a
ssDNA recombineering
In situ mutagenesis
Lactate
Online Access:http://link.springer.com/article/10.1186/s12934-019-1219-5

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http://link.springer.com/article/10.1186/s12934-019-1219-5

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