Polymerase chain reaction amplification and cloning of immunogenic protein NAD-dependent beta hydroxybutyryl CoA dehydrogenase gene of Clostridium chauvoei

Aim: The present study was aimed at polymerase chain reaction (PCR) amplification and cloning of NAD-dependent betahydroxybutyryl coenzyme A dehydrogenase (BHBD) gene of Clostridium chauvoei. Materials and Methods: C. chauvoei was cultured and confirmed by 16-23S rDNA spacer region primers. The pri...

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Bibliographic Details
Main Authors: Saroj K. Dangi, Ajay P. Singh, Satyaveer S. Dangi, Prasad Thomas, Santosh K. Gupta, Rajesh K. Agarwal, K. N. Viswas
Format: Article
Language:English
Published: Veterinary World 2014-10-01
Series:Veterinary World
Subjects:
Online Access:http://www.veterinaryworld.org/Vol.7/October-2014/18.pdf