Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue
Cell immobilisation in alcoholic fermentation has been extensively studied during the past few decades because of its technical and economical advantages over those of free cell systems. A biocatalyst was prepared by immobilising a commercial Saccharomyces cerevisiae strain (baker yeast) on corn ste...
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Matica srpska
2009-01-01
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Series: | Zbornik Matice Srpske za Prirodne Nauke |
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Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-4906/2009/0352-49060916315V.pdf |
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doaj-9e87e96be2b54afe817e2ea8fa52fed12021-01-02T03:41:09ZengMatica srpskaZbornik Matice Srpske za Prirodne Nauke0352-49062009-01-01200911631532210.2298/ZMSPN0916315VEthanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissueVučurović Vesna M.Razmovski Radojka N.Popov Stevan D.Cell immobilisation in alcoholic fermentation has been extensively studied during the past few decades because of its technical and economical advantages over those of free cell systems. A biocatalyst was prepared by immobilising a commercial Saccharomyces cerevisiae strain (baker yeast) on corn stem ground tissue for use in alcoholic fermentation. For this purpose, the yeast cells were submitted to the batch tests 'in situ' adsorption onto pieces of the corn stem ground tissue. Cells immobilisation was analysed by optical microscopy. It was determined that the addition of the corn stem ground tissue led to an increase of the pH value, total dissolved salts content, and sugar content in fermentation medium. The addition of 5 and 10g of the corn stem ground tissue per liter of medium, increased ethanol yield, decreased amount of residual sugar and the cells immobilisation was effective. Corn stem is one of the abundant, available, inexpensive, stable, reusable, nontoxic celulosic biomaterial with high porosity, which facilitates the transmission of substrates and products between carrier and medium. The prepared immobilised biocatalyst showed higher fermentation activity than free cells. The results indicate that corn stem might be an interesting support for yeast cell immobilisation, and also a cheap alternative recourse of mineral components with possibility of application for improving ethanol productivities. http://www.doiserbia.nb.rs/img/doi/0352-4906/2009/0352-49060916315V.pdfcorn stems ground tissueethanolfermentationimmobilizationyeast |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vučurović Vesna M. Razmovski Radojka N. Popov Stevan D. |
spellingShingle |
Vučurović Vesna M. Razmovski Radojka N. Popov Stevan D. Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue Zbornik Matice Srpske za Prirodne Nauke corn stems ground tissue ethanol fermentation immobilization yeast |
author_facet |
Vučurović Vesna M. Razmovski Radojka N. Popov Stevan D. |
author_sort |
Vučurović Vesna M. |
title |
Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue |
title_short |
Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue |
title_full |
Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue |
title_fullStr |
Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue |
title_full_unstemmed |
Ethanol production using Saccharomyces cerevisiae cells immobilised on corn stem ground tissue |
title_sort |
ethanol production using saccharomyces cerevisiae cells immobilised on corn stem ground tissue |
publisher |
Matica srpska |
series |
Zbornik Matice Srpske za Prirodne Nauke |
issn |
0352-4906 |
publishDate |
2009-01-01 |
description |
Cell immobilisation in alcoholic fermentation has been extensively studied during the past few decades because of its technical and economical advantages over those of free cell systems. A biocatalyst was prepared by immobilising a commercial Saccharomyces cerevisiae strain (baker yeast) on corn stem ground tissue for use in alcoholic fermentation. For this purpose, the yeast cells were submitted to the batch tests 'in situ' adsorption onto pieces of the corn stem ground tissue. Cells immobilisation was analysed by optical microscopy. It was determined that the addition of the corn stem ground tissue led to an increase of the pH value, total dissolved salts content, and sugar content in fermentation medium. The addition of 5 and 10g of the corn stem ground tissue per liter of medium, increased ethanol yield, decreased amount of residual sugar and the cells immobilisation was effective. Corn stem is one of the abundant, available, inexpensive, stable, reusable, nontoxic celulosic biomaterial with high porosity, which facilitates the transmission of substrates and products between carrier and medium. The prepared immobilised biocatalyst showed higher fermentation activity than free cells. The results indicate that corn stem might be an interesting support for yeast cell immobilisation, and also a cheap alternative recourse of mineral components with possibility of application for improving ethanol productivities. |
topic |
corn stems ground tissue ethanol fermentation immobilization yeast |
url |
http://www.doiserbia.nb.rs/img/doi/0352-4906/2009/0352-49060916315V.pdf |
work_keys_str_mv |
AT vucurovicvesnam ethanolproductionusingsaccharomycescerevisiaecellsimmobilisedoncornstemgroundtissue AT razmovskiradojkan ethanolproductionusingsaccharomycescerevisiaecellsimmobilisedoncornstemgroundtissue AT popovstevand ethanolproductionusingsaccharomycescerevisiaecellsimmobilisedoncornstemgroundtissue |
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