Mapping the Contact Sites of the Escherichia coli Division-Initiating Proteins FtsZ and ZapA by BAMG Cross-Linking and Site-Directed Mutagenesis

Mapping the Contact Sites of the Escherichia coli Division-Initiating Proteins FtsZ and ZapA by BAMG Cross-Linking and Site-Directed Mutagenesis

Cell division in bacteria is initiated by the polymerization of FtsZ at midcell in a ring-like structure called the Z-ring. ZapA and other proteins assist Z-ring formation and ZapA binds ZapB, which senses the presence of the nucleoids. The FtsZ–ZapA binding interface was analyzed by chemi...

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Bibliographic Details
Main Authors: Winfried Roseboom, Madhvi G. Nazir, Nils Y. Meiresonne, Tamimount Mohammadi, Jolanda Verheul, Hansuk Buncherd, Alexandre M. J. J. Bonvin, Leo J. de Koning, Chris G. de Koster, Luitzen de Jong, Tanneke den Blaauwen
Format: Article
Language:English
Published: MDPI AG 2018-09-01
Series:International Journal of Molecular Sciences
Subjects:
cell division
Z associated protein A (ZapA)
Filamenting temperature sensitive Z (FtsZ)
quadrupole time of flight mass spectrometer (QTOF)
Fourier-Transform Ion Cyclotron Resonance mass spectrometry(FTICR)
1,4-bis(succimidyl)-3-azidomethylglutarate (BAMG)
Online Access:http://www.mdpi.com/1422-0067/19/10/2928

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http://www.mdpi.com/1422-0067/19/10/2928

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