A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin

A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin

Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleav...

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Bibliographic Details
Main Authors: Shanshan Hou, Tingting Feng, Na Zhao, Jiaxin Zhang, Huibin Wang, Ning Liang, Longshan Zhao
Format: Article
Language:English
Published: Elsevier 2020-10-01
Series:Journal of Pharmaceutical Analysis
Subjects:
Carbon nanoparticles
Fluorescence quenching
Förster resonance energy transfer (FRET)
Fluorescein-labelled peptide
Trypsin assay
Online Access:http://www.sciencedirect.com/science/article/pii/S2095177920310510

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http://www.sciencedirect.com/science/article/pii/S2095177920310510

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