In silico screening of 393 mutants facilitates enzyme engineering of amidase activity in CalB
Our previously presented method for high throughput computational screening of mutant activity (Hediger et al., 2012) is benchmarked against experimentally measured amidase activity for 22 mutants of Candida antarctica lipase B (CalB). Using an appropriate cutoff criterion for the computed barriers,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2013-08-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/145.pdf |