An ELISA protocol to improve the accuracy and reliability of serological antibody assays

To assay serum antibodies by indirect ELISA, it is critical to eliminate a variety of false positive and negative reactions attributed to the principle. These include 1) the background (BG) noise reaction caused by hydrophobic binding of immunoglobulin components in sample specimens to solid surface...

Full description

Bibliographic Details
Main Authors: Takaki Waritani, Jessica Chang, Bonnie McKinney, Kuniaki Terato
Format: Article
Language:English
Published: Elsevier 2017-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016117300122