A novel 'gene insertion/marker out' (GIMO) method for transgene expression and gene complementation in rodent malaria parasites.

Research on the biology of malaria parasites has greatly benefited from the application of reverse genetic technologies, in particular through the analysis of gene deletion mutants and studies on transgenic parasites that express heterologous or mutated proteins. However, transfection in Plasmodium...

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Main Authors: Jing-wen Lin, Takeshi Annoura, Mohammed Sajid, Séverine Chevalley-Maurel, Jai Ramesar, Onny Klop, Blandine M D Franke-Fayard, Chris J Janse, Shahid M Khan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22216235/pdf/?tool=EBI