Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.

Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.

Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors w...

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Bibliographic Details
Main Authors: Shigeo S Sugano, Ryuichi Nishihama, Makoto Shirakawa, Junpei Takagi, Yoriko Matsuda, Sakiko Ishida, Tomoo Shimada, Ikuko Hara-Nishimura, Keishi Osakabe, Takayuki Kohchi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6209168?pdf=render

Internet

http://europepmc.org/articles/PMC6209168?pdf=render

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