Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes

Abstract Fluorescent sterol probes, comprising a fluorophore connected to a sterol backbone by means of a linker, are promising tools for enabling high-resolution imaging of intracellular cholesterol. In this study, we evaluated how the size of the linker, site of its attachment and nature of the fl...

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Bibliographic Details
Main Authors: Jarmila Králová, Michal Jurášek, Lucie Mikšátková, Anna Marešová, Jan Fähnrich, Petra Cihlářová, Pavel Drašar, Petr Bartůněk, Vladimír Král
Format: Article
Language:English
Published: Nature Publishing Group 2020-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-78085-9