Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites

Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites

Abstract Current genome walking methods are very time consuming, and many produce non-specific amplification products. To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR. This PCR...

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Bibliographic Details
Main Authors: Xianbo Jia, Xinjian Lin, Jichen Chen
Format: Article
Language:English
Published: SpringerOpen 2017-11-01
Series:AMB Express
Subjects:
LETAIL-PCR
Genome walking
Linear amplification
Tn5 transposon
Online Access:http://link.springer.com/article/10.1186/s13568-017-0495-x

Internet

http://link.springer.com/article/10.1186/s13568-017-0495-x

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