ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
We sought to develop a system that could increase the usefulness of oligonucleotide-mediated recombineering of bacterial chromosomes by expanding the types of modifications generated by an oligonucleotide (i.e., insertions and deletions) and by making recombinant formation a selectable event. This p...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2018-12-01
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Series: | mBio |
Subjects: | |
Online Access: | https://doi.org/10.1128/mBio.01467-18 |