ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

We sought to develop a system that could increase the usefulness of oligonucleotide-mediated recombineering of bacterial chromosomes by expanding the types of modifications generated by an oligonucleotide (i.e., insertions and deletions) and by making recombinant formation a selectable event. This p...

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Bibliographic Details
Main Authors: Kenan C. Murphy, Samantha J. Nelson, Subhalaxmi Nambi, Kadamba Papavinasasundaram, Christina E. Baer, Christopher M. Sassetti
Format: Article
Language:English
Published: American Society for Microbiology 2018-12-01
Series:mBio
Subjects:
Mycobacterium smegmatis
bacteriophage genetics
gene replacement
genetic fusions
metabolic engineering
promoter replacements
recombineering
tuberculosis
Online Access:https://doi.org/10.1128/mBio.01467-18

Internet

https://doi.org/10.1128/mBio.01467-18

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