Quantitative diffusion measurements using the open-source software PyFRAP

FRAP analysis often relies on simplified assumptions that can affect measurement accuracy. Here the authors present a Python-based FRAP analysis software using simulations instead of simplified theoretical models to fit the data, which accounts for complex sample geometries and bleach conditions.

Bibliographic Details
Main Authors: Alexander Bläßle, Gary Soh, Theresa Braun, David Mörsdorf, Hannes Preiß, Ben M. Jordan, Patrick Müller
Format: Article
Language:English
Published: Nature Publishing Group 2018-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03975-6