Using the LN34 Pan-Lyssavirus Real-Time RT-PCR Assay for Rabies Diagnosis and Rapid Genetic Typing from Formalin-Fixed Human Brain Tissue

Using the LN34 Pan-Lyssavirus Real-Time RT-PCR Assay for Rabies Diagnosis and Rapid Genetic Typing from Formalin-Fixed Human Brain Tissue

Human rabies post mortem diagnostic samples are often preserved in formalin. While immunohistochemistry (IHC) has been routinely used for rabies antigen detection in formalin-fixed tissue, the formalin fixation process causes nucleic acid fragmentation that may affect PCR amplification. This study r...

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Bibliographic Details
Main Authors: Rene Edgar Condori, Michael Niezgoda, Griselda Lopez, Carmen Acosta Matos, Elinna Diaz Mateo, Crystal Gigante, Claire Hartloge, Altagracia Pereira Filpo, Joseph Haim, Panayampalli Subbian Satheshkumar, Brett Petersen, Ryan Wallace, Victoria Olson, Yu Li
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Viruses
Subjects:
rabies virus
lyssavirus
diagnosis
ln34 assay
formalin-fixed tissue
genetic typing
Online Access:https://www.mdpi.com/1999-4915/12/1/120

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https://www.mdpi.com/1999-4915/12/1/120

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