Intra-domain phage display (ID-PhD) of peptides and protein mini-domains censored from canonical pIII phage display

Intra-domain phage display (ID-PhD) of peptides and protein mini-domains censored from canonical pIII phage display

In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage cloning vector, we introduced sites that perm...

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Bibliographic Details
Main Authors: Katrina F Tjhung, Frédérique eDeiss, Jessica eTran, Ying eChou, Ratmir eDerda
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-04-01
Series:Frontiers in Microbiology
Subjects:
Libraries
Peptides
Protein Engineering
Proteolysis
phage-display
in vitro selection
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00340/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00340/full

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