Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence

Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence

Summary: Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ...

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Bibliographic Details
Main Authors: Elizabeth K. Neumann, Nathan Heath Patterson, Jamie L. Allen, Lukasz G. Migas, Haichun Yang, Maya Brewer, David M. Anderson, Jennifer Harvey, Danielle B. Gutierrez, Raymond C. Harris, Mark P. deCaestecker, Agnes B. Fogo, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Health Sciences
Metabolomics
Microscopy
Antibody
Mass Spectrometry
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004548

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http://www.sciencedirect.com/science/article/pii/S2666166721004548

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