High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

Abstract Background Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated by protein factors, and this regulation can impact on the levels of miRN...

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Bibliographic Details
Main Authors: Jonathan Bouvette, Dursun Nizam Korkut, Aurélien Fouillen, Soumiya Amellah, Antonio Nanci, Yves Durocher, James G. Omichinski, Pascale Legault
Format: Article
Language:English
Published: BMC 2018-12-01
Series:BMC Biotechnology
Subjects:
Human HEK293-EBNA1 cells
Mammalian cell suspension culture
Dicer expression
Dicer purification
Pre-miRNA
Pre-let-7
Online Access:http://link.springer.com/article/10.1186/s12896-018-0485-3

Internet

http://link.springer.com/article/10.1186/s12896-018-0485-3

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