Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation
Two potent anti-MRSA tanshinone glycosides (1 and 2) were discovered by targeted microbial biotransformation, along with rapid identification via MS/MS networking. Serial reactions including dehydrogenation, demethylations, reduction, glycosylation and methylation have been observed after incubation...
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KeAi Communications Co., Ltd.
2016-09-01
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doaj-a11c33e1c62f44d1a13ee71538c484f22021-02-02T07:54:48ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2016-09-011318719410.1016/j.synbio.2016.05.002Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformationWenni He0Miaomiao Liu1Pei Huang2Wael M. Abdel-Mageed3Jianying Han4Jeramie D. Watrous5Don D. Nguyen6Wenzhao Wang7Fuhang Song8Huanqin Dai9Jingyu Zhang10Ronald J. Quinn11Tanja Grkovi12Houwei Luo13Lixin Zhang14Xueting Liu15Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaDepartment of Chemistry & Biochemistry, University of California San Diego, La Jolla, CA 92093, United StatesDepartment of Chemistry & Biochemistry, University of California San Diego, La Jolla, CA 92093, United StatesState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaEskitis Institute, Griffith University, Brisbane, QLD 4111, AustraliaEskitis Institute, Griffith University, Brisbane, QLD 4111, AustraliaDepartment of Natural Products, China Pharmaceutical University, Nanjing 210009, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaTwo potent anti-MRSA tanshinone glycosides (1 and 2) were discovered by targeted microbial biotransformation, along with rapid identification via MS/MS networking. Serial reactions including dehydrogenation, demethylations, reduction, glycosylation and methylation have been observed after incubation of tanshinone IIA and fungus Mucor rouxianus AS 3.3447. In addition, tanshinosides B (2) showed potent activities against serial clinical isolates of oxacillin-resistant Staphylococcus aureus with MIC values of 0.78 μg/mL. This is the first study that shows a significant increase in the level and activities of tanshinone glycosides relative to the substrate tanshinone IIA.http://www.sciencedirect.com/science/article/pii/S2405805X15300223BiotransformationGlycosylationTanshinone IIAMucor rouxianus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenni He Miaomiao Liu Pei Huang Wael M. Abdel-Mageed Jianying Han Jeramie D. Watrous Don D. Nguyen Wenzhao Wang Fuhang Song Huanqin Dai Jingyu Zhang Ronald J. Quinn Tanja Grkovi Houwei Luo Lixin Zhang Xueting Liu |
spellingShingle |
Wenni He Miaomiao Liu Pei Huang Wael M. Abdel-Mageed Jianying Han Jeramie D. Watrous Don D. Nguyen Wenzhao Wang Fuhang Song Huanqin Dai Jingyu Zhang Ronald J. Quinn Tanja Grkovi Houwei Luo Lixin Zhang Xueting Liu Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation Synthetic and Systems Biotechnology Biotransformation Glycosylation Tanshinone IIA Mucor rouxianus |
author_facet |
Wenni He Miaomiao Liu Pei Huang Wael M. Abdel-Mageed Jianying Han Jeramie D. Watrous Don D. Nguyen Wenzhao Wang Fuhang Song Huanqin Dai Jingyu Zhang Ronald J. Quinn Tanja Grkovi Houwei Luo Lixin Zhang Xueting Liu |
author_sort |
Wenni He |
title |
Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation |
title_short |
Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation |
title_full |
Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation |
title_fullStr |
Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation |
title_full_unstemmed |
Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation |
title_sort |
discovery of tanshinone derivatives with anti-mrsa activity via targeted bio-transformation |
publisher |
KeAi Communications Co., Ltd. |
series |
Synthetic and Systems Biotechnology |
issn |
2405-805X |
publishDate |
2016-09-01 |
description |
Two potent anti-MRSA tanshinone glycosides (1 and 2) were discovered by targeted microbial biotransformation, along with rapid identification via MS/MS networking. Serial reactions including dehydrogenation, demethylations, reduction, glycosylation and methylation have been observed after incubation of tanshinone IIA and fungus Mucor rouxianus AS 3.3447. In addition, tanshinosides B (2) showed potent activities against serial clinical isolates of oxacillin-resistant Staphylococcus aureus with MIC values of 0.78 μg/mL. This is the first study that shows a significant increase in the level and activities of tanshinone glycosides relative to the substrate tanshinone IIA. |
topic |
Biotransformation Glycosylation Tanshinone IIA Mucor rouxianus |
url |
http://www.sciencedirect.com/science/article/pii/S2405805X15300223 |
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