Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of <i>Aspergillus</i> Species in the Human Airway

Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of <i>Aspergillus</i> Species in the Human Airway

Background: Prior studies illustrate the presence and clinical importance of detecting <i>Aspergillus </i>species in the airways of patients with chronic respiratory disease. Despite this, a low fungal biomass and the presence of PCR inhibitors limits the usefulness of quantitative PCR (...

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Bibliographic Details
Main Authors: Tuang Yeow Poh, Nur A’tikah Binte Mohamed Ali, Louisa L. Y. Chan, Pei Yee Tiew, Sanjay H. Chotirmall
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:International Journal of Molecular Sciences
Subjects:
Aspergillus fumigatus
Aspergillus terreus
qPCR
ddPCR
quantification
Online Access:https://www.mdpi.com/1422-0067/21/9/3043

Internet

https://www.mdpi.com/1422-0067/21/9/3043

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