Expression cloning and production of Human Heavy Chain Only antibodies from murine transgenic plasma cells

Several technologies have been developed to isolate human antibodies against different target antigens as a source of potential therapeutics, including hybridoma technology, phage and yeast display systems. For conventional antibodies this involves either random pairing of VH and VL domains in combi...

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Bibliographic Details
Main Authors: Dubravka Drabek, Rick Janssens, Ernie de Boer, Rik Rademaker, Johannes Kloess, John Skehel, Frank Grosveld
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-12-01
Series:Frontiers in Immunology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00619/full