Expression cloning and production of Human Heavy Chain Only antibodies from murine transgenic plasma cells

Expression cloning and production of Human Heavy Chain Only antibodies from murine transgenic plasma cells

Several technologies have been developed to isolate human antibodies against different target antigens as a source of potential therapeutics, including hybridoma technology, phage and yeast display systems. For conventional antibodies this involves either random pairing of VH and VL domains in combi...

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Bibliographic Details
Main Authors: Dubravka Drabek, Rick Janssens, Ernie de Boer, Rik Rademaker, Johannes Kloess, John Skehel, Frank Grosveld
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-12-01
Series:Frontiers in Immunology
Subjects:
Plasma Cells
Transgenic mice
human Vh
HCAb
HEK cell library
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00619/full

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