Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry
Inspired by the many reported successful applications of DNA-encoded chemical libraries in drug discovery projects with protein targets, we decided to apply this platform to nucleic acid targets. We used a 120-billion-compound set of 33 distinct DNA-encoded chemical libraries and affinity-mediated s...
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doaj-a1b8a1bc30d44bde8fa1b458023345c82020-11-25T01:30:15ZengMDPI AGMolecules1420-30492019-05-012410202610.3390/molecules24102026molecules24102026Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded ChemistryAlexander Litovchick0Xia Tian1Michael I. Monteiro2Kaitlyn M. Kennedy3Marie-Aude Guié4Paolo Centrella5Ying Zhang6Matthew A. Clark7Anthony D. Keefe8X-Chem Pharmaceuticals, Waltham, MA 02435, USAArrakis Therapeutics, Waltham, MA 02451, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAX-Chem Pharmaceuticals, Waltham, MA 02435, USAInspired by the many reported successful applications of DNA-encoded chemical libraries in drug discovery projects with protein targets, we decided to apply this platform to nucleic acid targets. We used a 120-billion-compound set of 33 distinct DNA-encoded chemical libraries and affinity-mediated selection to discover binders to a panel of DNA targets. Here, we report the successful discovery of small molecules that specifically interacted with DNA G-quartets, which are stable structural motifs found in G-rich regions of genomic DNA, including in the promoter regions of oncogenes. For this study, we chose the G-quartet sequence found in the <i>c-myc</i> promoter as a primary target. Compounds enriched using affinity-mediated selection against this target demonstrated high-affinity binding and high specificity over DNA sequences not containing G-quartet motifs. These compounds demonstrated a moderate ability to discriminate between different G-quartet motifs and also demonstrated activity in a cell-based assay, suggesting direct target engagement in the cell. DNA-encoded chemical libraries and affinity-mediated selection are uniquely suited to discover binders to targets that have no inherent activity outside of a cellular context, and they may also be of utility in other nucleic acid structural motifs.https://www.mdpi.com/1420-3049/24/10/2026DNA-encoded chemical libraryaffinity-mediated selectionG-quartetc-mycSPR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexander Litovchick Xia Tian Michael I. Monteiro Kaitlyn M. Kennedy Marie-Aude Guié Paolo Centrella Ying Zhang Matthew A. Clark Anthony D. Keefe |
spellingShingle |
Alexander Litovchick Xia Tian Michael I. Monteiro Kaitlyn M. Kennedy Marie-Aude Guié Paolo Centrella Ying Zhang Matthew A. Clark Anthony D. Keefe Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry Molecules DNA-encoded chemical library affinity-mediated selection G-quartet c-myc SPR |
author_facet |
Alexander Litovchick Xia Tian Michael I. Monteiro Kaitlyn M. Kennedy Marie-Aude Guié Paolo Centrella Ying Zhang Matthew A. Clark Anthony D. Keefe |
author_sort |
Alexander Litovchick |
title |
Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry |
title_short |
Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry |
title_full |
Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry |
title_fullStr |
Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry |
title_full_unstemmed |
Novel Nucleic Acid Binding Small Molecules Discovered Using DNA-Encoded Chemistry |
title_sort |
novel nucleic acid binding small molecules discovered using dna-encoded chemistry |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-05-01 |
description |
Inspired by the many reported successful applications of DNA-encoded chemical libraries in drug discovery projects with protein targets, we decided to apply this platform to nucleic acid targets. We used a 120-billion-compound set of 33 distinct DNA-encoded chemical libraries and affinity-mediated selection to discover binders to a panel of DNA targets. Here, we report the successful discovery of small molecules that specifically interacted with DNA G-quartets, which are stable structural motifs found in G-rich regions of genomic DNA, including in the promoter regions of oncogenes. For this study, we chose the G-quartet sequence found in the <i>c-myc</i> promoter as a primary target. Compounds enriched using affinity-mediated selection against this target demonstrated high-affinity binding and high specificity over DNA sequences not containing G-quartet motifs. These compounds demonstrated a moderate ability to discriminate between different G-quartet motifs and also demonstrated activity in a cell-based assay, suggesting direct target engagement in the cell. DNA-encoded chemical libraries and affinity-mediated selection are uniquely suited to discover binders to targets that have no inherent activity outside of a cellular context, and they may also be of utility in other nucleic acid structural motifs. |
topic |
DNA-encoded chemical library affinity-mediated selection G-quartet c-myc SPR |
url |
https://www.mdpi.com/1420-3049/24/10/2026 |
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