Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue

Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue

Abstract Unlocking clinically translatable genomic information, including copy number alterations (CNA), from formalin-fixed paraffin-embedded (FFPE) tissue is challenging due to low yields and degraded DNA. We describe a robust, cost-effective low-coverage whole genome sequencing (LC WGS) method fo...

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Bibliographic Details
Main Authors: Tanjina Kader, David L. Goode, Stephen Q. Wong, Jacquie Connaughton, Simone M. Rowley, Lisa Devereux, David Byrne, Stephen B. Fox, Gisela Mir Arnau, Richard W. Tothill, Ian G. Campbell, Kylie L. Gorringe
Format: Article
Language:English
Published: BMC 2016-11-01
Series:Genome Medicine
Subjects:
Low coverage whole genome sequencing (LC WGS)
Low-input DNA
Copy number
Formalin-fixed paraffin-embedded (FFPE)
Next generation sequencing
Online Access:http://link.springer.com/article/10.1186/s13073-016-0375-z

Internet

http://link.springer.com/article/10.1186/s13073-016-0375-z

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