A 4-plex Droplet Digital PCR Method for Simultaneous Quantification and Differentiation of Pathogenic and Non-pathogenic Vibrio parahaemolyticus Based on Single Intact Cells

A 4-plex Droplet Digital PCR Method for Simultaneous Quantification and Differentiation of Pathogenic and Non-pathogenic Vibrio parahaemolyticus Based on Single Intact Cells

Vibrio parahaemolyticus is a significant seafood-borne pathogen, leading to serious acute gastrointestinal diseases worldwide. In this study, a reliable 4-plex droplet digital PCR (ddPCR) was successfully established and evaluated for the simultaneous detection of V. parahaemolyticus based on tlh, t...

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Bibliographic Details
Main Authors: Shuwen Lei, Xiaokui Gu, Wei Xue, Zhangquan Rong, Zhe Wang, Song Chen, Qingping Zhong
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Microbiology
Subjects:
Vibrio parahaemolyticus
4-plex ddPCR
qPCR
single intact cell
precise detection
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01727/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.01727/full

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