Fluorescence polarization and fluctuation analysis monitors subunit proximity, stoichiometry, and protein complex hydrodynamics.

Fluorescence polarization and fluctuation analysis monitors subunit proximity, stoichiometry, and protein complex hydrodynamics.

Förster resonance energy transfer (FRET) microscopy is frequently used to study protein interactions and conformational changes in living cells. The utility of FRET is limited by false positive and negative signals. To overcome these limitations we have developed Fluorescence Polarization and Fluctu...

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Bibliographic Details
Main Authors: Tuan A Nguyen, Pabak Sarkar, Jithesh V Veetil, Srinagesh V Koushik, Steven S Vogel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3364239?pdf=render

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http://europepmc.org/articles/PMC3364239?pdf=render

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