CD39 Expression Identifies Terminally Exhausted CD8+ T Cells.
Exhausted T cells express multiple co-inhibitory molecules that impair their function and limit immunity to chronic viral infection. Defining novel markers of exhaustion is important both for identifying and potentially reversing T cell exhaustion. Herein, we show that the ectonucleotidse CD39 is a...
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2015-10-01
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Series: | PLoS Pathogens |
Online Access: | http://europepmc.org/articles/PMC4618999?pdf=render |
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doaj-a33577d938d04eef98014e4ae8a823b42020-11-25T01:34:04ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742015-10-011110e100517710.1371/journal.ppat.1005177CD39 Expression Identifies Terminally Exhausted CD8+ T Cells.Prakash K GuptaJernej GodecDavid WolskiEmily AdlandKathleen YatesKristen E PaukenCormac CosgroveCarola LedderoseWolfgang G JungerSimon C RobsonE John WherryGalit AlterPhilip J R GoulderPaul KlenermanArlene H SharpeGeorg M LauerW Nicholas HainingExhausted T cells express multiple co-inhibitory molecules that impair their function and limit immunity to chronic viral infection. Defining novel markers of exhaustion is important both for identifying and potentially reversing T cell exhaustion. Herein, we show that the ectonucleotidse CD39 is a marker of exhausted CD8+ T cells. CD8+ T cells specific for HCV or HIV express high levels of CD39, but those specific for EBV and CMV do not. CD39 expressed by CD8+ T cells in chronic infection is enzymatically active, co-expressed with PD-1, marks cells with a transcriptional signature of T cell exhaustion and correlates with viral load in HIV and HCV. In the mouse model of chronic Lymphocytic Choriomeningitis Virus infection, virus-specific CD8+ T cells contain a population of CD39high CD8+ T cells that is absent in functional memory cells elicited by acute infection. This CD39high CD8+ T cell population is enriched for cells with the phenotypic and functional profile of terminal exhaustion. These findings provide a new marker of T cell exhaustion, and implicate the purinergic pathway in the regulation of T cell exhaustion.http://europepmc.org/articles/PMC4618999?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Prakash K Gupta Jernej Godec David Wolski Emily Adland Kathleen Yates Kristen E Pauken Cormac Cosgrove Carola Ledderose Wolfgang G Junger Simon C Robson E John Wherry Galit Alter Philip J R Goulder Paul Klenerman Arlene H Sharpe Georg M Lauer W Nicholas Haining |
spellingShingle |
Prakash K Gupta Jernej Godec David Wolski Emily Adland Kathleen Yates Kristen E Pauken Cormac Cosgrove Carola Ledderose Wolfgang G Junger Simon C Robson E John Wherry Galit Alter Philip J R Goulder Paul Klenerman Arlene H Sharpe Georg M Lauer W Nicholas Haining CD39 Expression Identifies Terminally Exhausted CD8+ T Cells. PLoS Pathogens |
author_facet |
Prakash K Gupta Jernej Godec David Wolski Emily Adland Kathleen Yates Kristen E Pauken Cormac Cosgrove Carola Ledderose Wolfgang G Junger Simon C Robson E John Wherry Galit Alter Philip J R Goulder Paul Klenerman Arlene H Sharpe Georg M Lauer W Nicholas Haining |
author_sort |
Prakash K Gupta |
title |
CD39 Expression Identifies Terminally Exhausted CD8+ T Cells. |
title_short |
CD39 Expression Identifies Terminally Exhausted CD8+ T Cells. |
title_full |
CD39 Expression Identifies Terminally Exhausted CD8+ T Cells. |
title_fullStr |
CD39 Expression Identifies Terminally Exhausted CD8+ T Cells. |
title_full_unstemmed |
CD39 Expression Identifies Terminally Exhausted CD8+ T Cells. |
title_sort |
cd39 expression identifies terminally exhausted cd8+ t cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2015-10-01 |
description |
Exhausted T cells express multiple co-inhibitory molecules that impair their function and limit immunity to chronic viral infection. Defining novel markers of exhaustion is important both for identifying and potentially reversing T cell exhaustion. Herein, we show that the ectonucleotidse CD39 is a marker of exhausted CD8+ T cells. CD8+ T cells specific for HCV or HIV express high levels of CD39, but those specific for EBV and CMV do not. CD39 expressed by CD8+ T cells in chronic infection is enzymatically active, co-expressed with PD-1, marks cells with a transcriptional signature of T cell exhaustion and correlates with viral load in HIV and HCV. In the mouse model of chronic Lymphocytic Choriomeningitis Virus infection, virus-specific CD8+ T cells contain a population of CD39high CD8+ T cells that is absent in functional memory cells elicited by acute infection. This CD39high CD8+ T cell population is enriched for cells with the phenotypic and functional profile of terminal exhaustion. These findings provide a new marker of T cell exhaustion, and implicate the purinergic pathway in the regulation of T cell exhaustion. |
url |
http://europepmc.org/articles/PMC4618999?pdf=render |
work_keys_str_mv |
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