Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

Abstract Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in...

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Bibliographic Details
Main Authors: Francesco Sambo, Francesca Finotello, Enrico Lavezzo, Giacomo Baruzzo, Giulia Masi, Elektra Peta, Marco Falda, Stefano Toppo, Luisa Barzon, Barbara Di Camillo
Format: Article
Language:English
Published: BMC 2018-09-01
Series:BMC Bioinformatics
Subjects:
16S rRNA sequencing
Primer design
Multi objective optimization
Online Access:http://link.springer.com/article/10.1186/s12859-018-2360-6

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http://link.springer.com/article/10.1186/s12859-018-2360-6

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