Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis Anticorpo anti-intimina: caracterização imunológica e diagnóstico de amostras de EPEC e EHEC

Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis Anticorpo anti-intimina: caracterização imunológica e diagnóstico de amostras de EPEC e EHEC

Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin r...

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Bibliographic Details
Main Authors: Paula Célia Mariko Koga, Caroline Anunciação Menezes, Flávia Afonso Lima, Júlia Mitico Nara, Caroline Arantes Magalhães, Aurora Marques Cianciarullo, Jorge Mario da Costa Ferreira-Júnior, Luiz Rachid Trabulsi, Meire Roberta Bresciani Mendes-Ledesma, Roxane Maria Fontes Piazza
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2003-11-01
Series:Brazilian Journal of Microbiology
Subjects:
intimina
análise fenotípica
anticorpo policlonal
EPEC
EHEC
intimin
phenotypical analysis
polyclonal antibody
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002

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