A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be image...

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Bibliographic Details
Main Authors: Sonal Nagarkar-Jaiswal, Pei-Tseng Lee, Megan E Campbell, Kuchuan Chen, Stephanie Anguiano-Zarate, Manuel Cantu Gutierrez, Theodore Busby, Wen-Wen Lin, Yuchun He, Karen L Schulze, Benjamin W Booth, Martha Evans-Holm, Koen JT Venken, Robert W Levis, Allan C Spradling, Roger A Hoskins, Hugo J Bellen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2015-03-01
Series:eLife
Subjects:
RMCE
deGradFP
iGFPi
bruchpilot
discs large 1
dunce
Online Access:https://elifesciences.org/articles/05338

Internet

https://elifesciences.org/articles/05338

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