A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction

A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction

MicroED has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nanometers in thickness. Furthermore, traditional micr...

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Bibliographic Details
Main Authors: Emma V. Beale, David G. Waterman, Corey Hecksel, Jason van Rooyen, James B. Gilchrist, James M. Parkhurst, Felix de Haas, Bart Buijsse, Gwyndaf Evans, Peijun Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Molecular Biosciences
Subjects:
microED
cryoFIB
lamella
proteinase K
nanocrystals
cryoEM
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2020.00179/full

Internet

https://www.frontiersin.org/article/10.3389/fmolb.2020.00179/full

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