Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA....
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002215 |