Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach
Lipid accumulation is the hallmark of Non-alcoholic Fatty Liver Disease (NAFLD) and has been suggested to play a role in promoting fatty liver inflammation. Previous findings indicate that during oxidative stress conditions excess cholesterol autoxidizes to oxysterols. To date, the role of oxysterol...
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doaj-a37f05cc7f0c4ae6bd176ec215b672622020-11-24T20:42:53ZengElsevierRedox Biology2213-23172016-10-019C29630510.1016/j.redox.2016.09.002Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approachGaetano Serviddio0Francesco Bellanti1Rosanna Villani2Rosanna Tamborra3Chiara Zerbinati4Maria Blonda5Marco Ciacciarelli6Giuseppe Poli7Gianluigi Vendemiale8Luigi Iuliano9CURE University Centre for Liver Disease Research and Treatment, Institute of Internal Medicine, Department of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyCURE University Centre for Liver Disease Research and Treatment, Institute of Internal Medicine, Department of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyCURE University Centre for Liver Disease Research and Treatment, Institute of Internal Medicine, Department of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyCURE University Centre for Liver Disease Research and Treatment, Institute of Internal Medicine, Department of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyLaboratory of Vascular Biology and Mass Spectrometry, Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyCURE University Centre for Liver Disease Research and Treatment, Institute of Internal Medicine, Department of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyLaboratory of Vascular Biology and Mass Spectrometry, Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyDepartment of Clinical and Biological Sciences, University of Torino at San Luigi Gonzaga Hospital, 10043 Torino, Orbassano, ItalyCURE University Centre for Liver Disease Research and Treatment, Institute of Internal Medicine, Department of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyLaboratory of Vascular Biology and Mass Spectrometry, Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyLipid accumulation is the hallmark of Non-alcoholic Fatty Liver Disease (NAFLD) and has been suggested to play a role in promoting fatty liver inflammation. Previous findings indicate that during oxidative stress conditions excess cholesterol autoxidizes to oxysterols. To date, the role of oxysterols and their potential interaction with fatty acids accumulation in NASH pathogenesis remains little investigated. We used the nutritional model of high fatty acids (HFA), high cholesterol (HCh) or high fat and high cholesterol (HFA+FCh) diets and explored by a lipidomic approach, the blood and liver distribution of fatty acids and oxysterols in response to dietary manipulation. We observed that HFA or HCh diets induced fatty liver without inflammation, which was otherwise observed only after supplementation of HFA+HCh. Very interestingly, the combination model was associated with a specific oxysterol fingerprint. The present work provides a complete analysis of the change in lipids and oxysterols profile induced by different lipid dietary model and their association with histological alteration of the liver. This study allows the generation of interesting hypotheses on the role of interaction of lipid and cholesterol metabolites in the liver injury during NAFLD development and progression. Moreover, the changes in the concentration and quality of oxysterols induced by a combination diet suggest a novel potential pathogenic mechanism in the progression from simple steatosis to steatohepatitis.http://www.sciencedirect.com/science/article/pii/S2213231716300660OxysterolsNon-alcoholic fatty liver diseaseCholesterol excessFatty acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaetano Serviddio Francesco Bellanti Rosanna Villani Rosanna Tamborra Chiara Zerbinati Maria Blonda Marco Ciacciarelli Giuseppe Poli Gianluigi Vendemiale Luigi Iuliano |
spellingShingle |
Gaetano Serviddio Francesco Bellanti Rosanna Villani Rosanna Tamborra Chiara Zerbinati Maria Blonda Marco Ciacciarelli Giuseppe Poli Gianluigi Vendemiale Luigi Iuliano Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach Redox Biology Oxysterols Non-alcoholic fatty liver disease Cholesterol excess Fatty acids |
author_facet |
Gaetano Serviddio Francesco Bellanti Rosanna Villani Rosanna Tamborra Chiara Zerbinati Maria Blonda Marco Ciacciarelli Giuseppe Poli Gianluigi Vendemiale Luigi Iuliano |
author_sort |
Gaetano Serviddio |
title |
Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach |
title_short |
Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach |
title_full |
Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach |
title_fullStr |
Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach |
title_full_unstemmed |
Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach |
title_sort |
effects of dietary fatty acids and cholesterol excess on liver injury: a lipidomic approach |
publisher |
Elsevier |
series |
Redox Biology |
issn |
2213-2317 |
publishDate |
2016-10-01 |
description |
Lipid accumulation is the hallmark of Non-alcoholic Fatty Liver Disease (NAFLD) and has been suggested to play a role in promoting fatty liver inflammation. Previous findings indicate that during oxidative stress conditions excess cholesterol autoxidizes to oxysterols. To date, the role of oxysterols and their potential interaction with fatty acids accumulation in NASH pathogenesis remains little investigated.
We used the nutritional model of high fatty acids (HFA), high cholesterol (HCh) or high fat and high cholesterol (HFA+FCh) diets and explored by a lipidomic approach, the blood and liver distribution of fatty acids and oxysterols in response to dietary manipulation. We observed that HFA or HCh diets induced fatty liver without inflammation, which was otherwise observed only after supplementation of HFA+HCh. Very interestingly, the combination model was associated with a specific oxysterol fingerprint.
The present work provides a complete analysis of the change in lipids and oxysterols profile induced by different lipid dietary model and their association with histological alteration of the liver. This study allows the generation of interesting hypotheses on the role of interaction of lipid and cholesterol metabolites in the liver injury during NAFLD development and progression. Moreover, the changes in the concentration and quality of oxysterols induced by a combination diet suggest a novel potential pathogenic mechanism in the progression from simple steatosis to steatohepatitis. |
topic |
Oxysterols Non-alcoholic fatty liver disease Cholesterol excess Fatty acids |
url |
http://www.sciencedirect.com/science/article/pii/S2213231716300660 |
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