An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid, portable and accurate features. However, cleavage of the amplicons and primers by the cis- and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single reactio...

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Bibliographic Details
Main Authors: Jiaojiao Gong, Lijuan Kan, Xiuming Zhang, Ying He, Jiaqiang Pan, Liping Zhao, Qianyun Li, Menghao Liu, Jie Tian, Sili Lin, Zhouyu Lu, Liang Xue, Chaojun Wang, Guanghui Tang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2021-12-01
Series:Bioactive Materials
Subjects:
CRISPR-Cas12a
Onepot
Visual detection
Online Access:http://www.sciencedirect.com/science/article/pii/S2452199X21002255

Internet

http://www.sciencedirect.com/science/article/pii/S2452199X21002255

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