A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour
Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil...
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Sociedade Brasileira de Microbiologia
2016-03-01
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doaj-a3c829a5c6e044e4a708f0fdee26101a2020-11-25T00:42:26ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052016-03-0147112012810.1016/j.bjm.2015.11.018S1517-83822016000100120A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flourSheila Lorena de Araújo CoelhoValter Cruz MagalhãesPhellippe Arthur Santos MarbachMarcia Luciana CazettaAbstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was stimulated in the presence of Mg+2, Ca+2, EDTA, K+, Ba+2 and Na+ and inhibited in the presence of Hg+2, Cu+2, Fe+2 and Zn+2. The results showed that Bacillus sp. SM-02 have good potential for β-CGTase production.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100120&lng=en&tlng=enMicrobial enzymeFermentationAgro-industrial substrates |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sheila Lorena de Araújo Coelho Valter Cruz Magalhães Phellippe Arthur Santos Marbach Marcia Luciana Cazetta |
spellingShingle |
Sheila Lorena de Araújo Coelho Valter Cruz Magalhães Phellippe Arthur Santos Marbach Marcia Luciana Cazetta A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour Brazilian Journal of Microbiology Microbial enzyme Fermentation Agro-industrial substrates |
author_facet |
Sheila Lorena de Araújo Coelho Valter Cruz Magalhães Phellippe Arthur Santos Marbach Marcia Luciana Cazetta |
author_sort |
Sheila Lorena de Araújo Coelho |
title |
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour |
title_short |
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour |
title_full |
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour |
title_fullStr |
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour |
title_full_unstemmed |
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour |
title_sort |
new alkalophilic isolate of bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2016-03-01 |
description |
Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was stimulated in the presence of Mg+2, Ca+2, EDTA, K+, Ba+2 and Na+ and inhibited in the presence of Hg+2, Cu+2, Fe+2 and Zn+2. The results showed that Bacillus sp. SM-02 have good potential for β-CGTase production. |
topic |
Microbial enzyme Fermentation Agro-industrial substrates |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100120&lng=en&tlng=en |
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