Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products.

Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products.

We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina paired-end protocol to generate millions of overlapping reads. Combinatorial sequ...

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Bibliographic Details
Main Authors: Gregory B Gloor, Ruben Hummelen, Jean M Macklaim, Russell J Dickson, Andrew D Fernandes, Roderick MacPhee, Gregor Reid
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-10-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2964327?pdf=render

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http://europepmc.org/articles/PMC2964327?pdf=render

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