Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research

Abstract The global incidence of obesity has led to an increasing need for understanding the molecular mechanisms that drive this epidemic and its comorbidities. Quantitative real-time RT-PCR (RT-qPCR) is the most reliable and widely used method for gene expression analysis. The selection of suitabl...

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Bibliographic Details
Main Authors: Lester J. Perez, Liliam Rios, Purvi Trivedi, Kenneth D’Souza, Andrew Cowie, Carine Nzirorera, Duncan Webster, Keith Brunt, Jean-Francois Legare, Ansar Hassan, Petra C. Kienesberger, Thomas Pulinilkunnil
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-03730-9