Forward design of a complex enzyme cascade reaction

Building multi-component enzymatic processes in one pot is challenged by the inherent complexity of each biochemical system. Here, the authors use online mass spectroscopy and engineering systems theory to achieve forward design of a ten-membered reaction cascade.

Bibliographic Details
Main Authors: Christoph Hold, Sonja Billerbeck, Sven Panke
Format: Article
Language:English
Published: Nature Publishing Group 2016-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms12971