Metagenomic Information Recovery from Human Stool Samples Is Influenced by Sequencing Depth and Profiling Method

Metagenomic Information Recovery from Human Stool Samples Is Influenced by Sequencing Depth and Profiling Method

Sequencing of the 16S rRNA gene (16S) has long been a go-to method for microbiome characterization due to its accessibility and lower cost compared to shotgun metagenomic sequencing (SMS). However, 16S sequencing rarely provides species-level resolution and cannot provide direct assessment of other...

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Bibliographic Details
Main Authors: Tasha M. Santiago-Rodriguez, Aaron Garoutte, Emmase Adams, Waleed Nasser, Matthew C. Ross, Alex La Reau, Zachariah Henseler, Tonya Ward, Dan Knights, Joseph F. Petrosino, Emily B. Hollister
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Genes
Subjects:
alignment
marker gene
microbiome
shallow sequencing
shotgun metagenomic sequencing
virome
Online Access:https://www.mdpi.com/2073-4425/11/11/1380

Internet

https://www.mdpi.com/2073-4425/11/11/1380

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