TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR.

The advent of fluorescence-based quantitative real-time PCR (qPCR) has revolutionized the quantification of gene expression analysis in many fields, including life sciences, agriculture, forensic science, molecular diagnostics, and medicine. While SYBR Green-based qPCR is the most commonly-used plat...

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Bibliographic Details
Main Authors: Qian Zhang, Jing Wang, Fang Deng, Zhengjian Yan, Yinglin Xia, Zhongliang Wang, Jixing Ye, Youlin Deng, Zhonglin Zhang, Min Qiao, Ruifang Li, Sahitya K Denduluri, Qiang Wei, Lianggong Zhao, Shun Lu, Xin Wang, Shengli Tang, Hao Liu, Hue H Luu, Rex C Haydon, Tong-Chuan He, Li Jiang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4501803?pdf=render