LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites

LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites

Retrotransposons are abundant in mammalian genomes and can modulate the gene expression of surrounding genes by disrupting endogenous binding sites for transcription factors (TFs) or providing novel TFs binding sites within retrotransposon sequences. Here, we show that a (C/T)CACACCT sequence motif...

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Bibliographic Details
Main Authors: Yukuto Yasuhiko, Yoko Hirabayashi, Ryuichi Ono
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-06-01
Series:Frontiers in Chemistry
Subjects:
endogenous retroviruses
retrotransposon
transcription factors
evolution
TBX6
Online Access:http://journal.frontiersin.org/article/10.3389/fchem.2017.00034/full
Description
Summary:Retrotransposons are abundant in mammalian genomes and can modulate the gene expression of surrounding genes by disrupting endogenous binding sites for transcription factors (TFs) or providing novel TFs binding sites within retrotransposon sequences. Here, we show that a (C/T)CACACCT sequence motif in ORR1A, ORR1B, ORR1C, and ORR1D, Long Terminal Repeats (LTRs) of MaLR endogenous retrovirus (ERV), is the direct target of Tbx6, an evolutionary conserved family of T-box TFs. Moreover, by comparing gene expression between control mice (Tbx6 +/−) and Tbx6-deficient mice (Tbx6 −/−), we demonstrate that at least four genes, Twist2, Pitx2, Oscp1, and Nfxl1, are down-regulated with Tbx6 deficiency. These results suggest that ORR1A, ORR1B, ORR1C and ORR1D may contribute to the evolution of mammalian embryogenesis.
ISSN:2296-2646

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