LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites
Retrotransposons are abundant in mammalian genomes and can modulate the gene expression of surrounding genes by disrupting endogenous binding sites for transcription factors (TFs) or providing novel TFs binding sites within retrotransposon sequences. Here, we show that a (C/T)CACACCT sequence motif...
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doaj-a67a59c32d134c5a8266b0db79cd98e82020-11-24T21:01:39ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462017-06-01510.3389/fchem.2017.00034250166LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding SitesYukuto YasuhikoYoko HirabayashiRyuichi OnoRetrotransposons are abundant in mammalian genomes and can modulate the gene expression of surrounding genes by disrupting endogenous binding sites for transcription factors (TFs) or providing novel TFs binding sites within retrotransposon sequences. Here, we show that a (C/T)CACACCT sequence motif in ORR1A, ORR1B, ORR1C, and ORR1D, Long Terminal Repeats (LTRs) of MaLR endogenous retrovirus (ERV), is the direct target of Tbx6, an evolutionary conserved family of T-box TFs. Moreover, by comparing gene expression between control mice (Tbx6 +/−) and Tbx6-deficient mice (Tbx6 −/−), we demonstrate that at least four genes, Twist2, Pitx2, Oscp1, and Nfxl1, are down-regulated with Tbx6 deficiency. These results suggest that ORR1A, ORR1B, ORR1C and ORR1D may contribute to the evolution of mammalian embryogenesis.http://journal.frontiersin.org/article/10.3389/fchem.2017.00034/fullendogenous retrovirusesretrotransposontranscription factorsevolutionTBX6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yukuto Yasuhiko Yoko Hirabayashi Ryuichi Ono |
spellingShingle |
Yukuto Yasuhiko Yoko Hirabayashi Ryuichi Ono LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites Frontiers in Chemistry endogenous retroviruses retrotransposon transcription factors evolution TBX6 |
author_facet |
Yukuto Yasuhiko Yoko Hirabayashi Ryuichi Ono |
author_sort |
Yukuto Yasuhiko |
title |
LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites |
title_short |
LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites |
title_full |
LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites |
title_fullStr |
LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites |
title_full_unstemmed |
LTRs of Endogenous Retroviruses as a Source of Tbx6 Binding Sites |
title_sort |
ltrs of endogenous retroviruses as a source of tbx6 binding sites |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Chemistry |
issn |
2296-2646 |
publishDate |
2017-06-01 |
description |
Retrotransposons are abundant in mammalian genomes and can modulate the gene expression of surrounding genes by disrupting endogenous binding sites for transcription factors (TFs) or providing novel TFs binding sites within retrotransposon sequences. Here, we show that a (C/T)CACACCT sequence motif in ORR1A, ORR1B, ORR1C, and ORR1D, Long Terminal Repeats (LTRs) of MaLR endogenous retrovirus (ERV), is the direct target of Tbx6, an evolutionary conserved family of T-box TFs. Moreover, by comparing gene expression between control mice (Tbx6 +/−) and Tbx6-deficient mice (Tbx6 −/−), we demonstrate that at least four genes, Twist2, Pitx2, Oscp1, and Nfxl1, are down-regulated with Tbx6 deficiency. These results suggest that ORR1A, ORR1B, ORR1C and ORR1D may contribute to the evolution of mammalian embryogenesis. |
topic |
endogenous retroviruses retrotransposon transcription factors evolution TBX6 |
url |
http://journal.frontiersin.org/article/10.3389/fchem.2017.00034/full |
work_keys_str_mv |
AT yukutoyasuhiko ltrsofendogenousretrovirusesasasourceoftbx6bindingsites AT yokohirabayashi ltrsofendogenousretrovirusesasasourceoftbx6bindingsites AT ryuichiono ltrsofendogenousretrovirusesasasourceoftbx6bindingsites |
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1716777407266947072 |