Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR). Here, we used CRISPR/Cas9 to generate targeted double-strand br...

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Bibliographic Details
Main Authors: Haroon Butt, Ayman Eid, Zahir Ali, Mohamed A. M. Atia, Morad M. Mokhtar, Norhan Hassan, Ciaran M. Lee, Gang Bao, Magdy M. Mahfouz
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-08-01
Series:Frontiers in Plant Science
Subjects:
genome engineering
CRISPR/Cas9
HDR
RNA-templated repair
gene editing
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01441/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2017.01441/full

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