Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from Leishmania infantum for in vitro expression screening
We describe a streamlined reverse transcription-polymerase chain reaction methodology for constructing full-length cDNA libraries of trypanosomatids on the basis of conserved sequences located at the 5' and 3'ends of trans-spliced mRNAs. The amplified cDNA corresponded to full-length messe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2003-06-01
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| Series: | Memórias do Instituto Oswaldo Cruz. |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000400008 |