In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active mole...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-02-01
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| Series: | Biology |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-7737/3/1/167 |