Use of amplicon melt temperature to detect SARS-CoV-2 Lineage B.1.1.7 variant through the G28048T mutation in the ORF 8 gene

Use of amplicon melt temperature to detect SARS-CoV-2 Lineage B.1.1.7 variant through the G28048T mutation in the ORF 8 gene

A new variant of SARS-CoV-2 (Lineage B.1.1.7) was identified in the UK in December 2020 which was associated with higher transmissibility of COVID-19. The AusDiagnostics SARS-CoV-2, Influenza and RSV 8-well assay is used at sixteen UK hospitals and detects part of the ORF8 gene (together with a segm...

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Bibliographic Details
Main Authors: Richard S. Hale, Ashleigh Green, Emma Cunningham, Joel Paul
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Journal of Clinical Virology Plus
Subjects:
SARS-Cov-2 Lineage B.1.1.7
Variant detection
Molecular diagnostics
MT-PCR
Online Access:http://www.sciencedirect.com/science/article/pii/S266703802100017X

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http://www.sciencedirect.com/science/article/pii/S266703802100017X

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